Hallo Sorjs,
I used Relion 1.2 for a reconstruction from about 150000 single particle images. The calculations took a while to finish and I have a few question regarding the speed up of the calculations:
- Relion did note made us of all the RAM available. I tried to increase the memory limit by setting --max_memory is there anything else I have to do?
- The 2D classification of the data set was very slow and I had to skip this step. The MPI OMP combination did not work (the classification was started 20 times independently, I think). The 3D classification worked. Could this step be speed up by initially using down sampled images?
Thank you for your help,
Daniel
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