George, would you please explain your comments? We've found the TA
Instruments analysis software very robust and user friendly.
We have the low volume nanoITC from TA instruments and get equivalent #'s
in our comparison tests to the Microcal instrument.
Cheers,
Chris
--
Christopher L. Colbert, Ph.D.
Assistant Professor
Department of Chemistry and Biochemistry
North Dakota State University
P.O. Box 6050 Dept. 2710
Fargo, ND 58108-6050
PH: (701) 231-7946
FAX: (701) 231-8324
On 3/23/13 8:47 AM, "George Kontopidis" <[log in to unmask]> wrote:
>Keep in mind that output files from nanoITC, TA instrument cannot be red
>by
>Origin. At some point you will need to analyse your data further.
>
>George
>
>-----Original Message-----
>From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
>Anastassis Perrakis
>Sent: Saturday, March 23, 2013 12:46 PM
>To: [log in to unmask]
>Subject: Re: [ccp4bb] Isothermal titration calorimetry
>
>It might be worth to consider the question more in detail.
>
>Do you want to study thermodynamics of the interaction, or a KD would do?
>If
>the former, you need ITC. If the latter, and you want to study things at
>the
>level of KD only, maybe investing on a plate reader, thermophoresis, or
>some
>biosensor technology (spr or interferometry based systems) should be
>considered.
>
>Then, what interactions will you study with the ITC? In general, I would
>agree that the lower sample volume is worth the nano options, but
>depending
>on the typical systems under study, sometimes the gain on sample quantity
>is
>not worth the money - while many times its worth it.
>
>John is if course right that for studying specific systems as the one he
>describes the 200 is great.
>
>A.
>
>Sent from my iPhone
>
>On 23 Mar 2013, at 11:00, John Fisher <[log in to unmask]> wrote:
>
>> I would recommend the Microcal ITC 200, hands down. Not only is it an
>amazing instrument with the optional automated sample loader (which is
>worth
>every penny), but we were able to do experiments (multiple) using
>FULL-LENGTH p53 binding to a weak cognate protein. I believe this was the
>first time ITC was ever used with full length p53, as it is so labile and
>just loves immediately to oligomerize. Sample sizes pay for the
>instrument.
>> Best,
>> John
>>
>> John Fisher, M.D./PhD
>> St. Jude Children's Research Hospital
>> Department of Oncology
>> Department of Structural Biology
>> W: 901-595-6193
>> C: 901-409-5699
>>
>> On Mar 23, 2013, at 4:45 AM, Sameh Soror <[log in to unmask]>
>>wrote:
>>
>>> Dear All,
>>>
>>>
>>> I am sorry for the off topic question. I am going to buy ITC to study
>protein-protein & protein-ligand interactions....
>>>
>>> I am comparing microcal, GE and nanoITC, TA instrument..
>>> any suggestions, recommendations, good experiences or bad experiences.
>>>is
>there a better system.
>>>
>>>
>>> Thank in advance for the help.
>>>
>>>
>>> Regards
>>>
>>>
>>> Sameh
>>>
>>> --
>>> Sameh Soror
>>>
>>> Postdoc. fellow
>>>
>>>
>
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