Dear all
I have a His-tagged membrane protein with unknown oligomerization state. But I am worried that tag addition may induce different state than in native and affect its crystallizability.
Is there a single method that can determine the oligomerization state with nearly 100% accuracy? I have use of AUC and SAXS but there seems to be ambiguity about detergent and lipid effects. Is Thermofluor a right method?
Does oligomerization require special assembly proteins, which will mean that tag cleavage is not useful to obtain native state?
Thank you.
Theresa
|