Dear James,
I agree with your chronology of the first full new protein structures by SR MAD.
The 1975 two wavelength Hoppe and Jakubowksi study of erythrocruorin with Ni and Co Kalpha Xray tubes is a classic piece of work of in effect MAD phasing . See the IUCr Anomalous Scattering Conference book edited by Abrahams and Ramaseshan.
The 1971 Nature paper biological diffraction with SR from Hamburg, whose focus was on muscle diffraction, which Colin highlighted, does have an entry though for a 'protein crystal' in a table.
There is also of course the Hamburg 1976 paper Harmsen et al J Mol Biol but which generally concluded SR for protein crystallography wasn't worth it; to me as a doctoral student at the time this was clearly an incorrect conclusion I firmly believed based on the 1971 Hamburg Nature paper and especially what I could see in and beyond the 1976 SSRL PNAS paper.
Yours sincerely,
John
Prof John R Helliwell DSc
On 16 Mar 2013, at 14:46, James Holton <[log in to unmask]> wrote:
> The first report of shooting a protein crystal at a synchrotron (I think) was in 1976:
> http://www.pnas.org/content/73/1/128.full.pdf
> that was rubredoxin
>
> The first PDB file that contains a "SYNCHROTRON=Y" entry is 1tld (trypsin), which was deposited in 1989:
> http://dx.doi.org/10.1016/0022-2836(89)90110-1
> But the structure of trypsin was arguably already "solved" at that time.
> Anomalous diffraction was first demonstrated by Coster, Knoll and Prins in 1930
> http://dx.doi.org/10.1007/BF01339610
> this was 20 years before Bijvoet. But not with a synchrotron and definitely not with a protein
>
> The first protein to be solved using anomalous was crambin in 1981:
> http://dx.doi.org/10.1038/290107a0
> but this was not using a synchrotron
>
> The first demonstration of MAD on a protein at a synchrotron was a Tb soak of parvalbumin in 1985
> http://dx.doi.org/10.1016/0014-5793(85)80207-6
> but one could argue that several parvalbumins were already known at that time.
>
> The first MAD structure from native metals was cucumber blue copper protein (2cbp) in 1989
> http://dx.doi.org/10.1126%2Fscience.3406739
>
> The first "new" structure using MAD, as well as the first SeMet was ribonuclease H (1rnh) in 1990
> http://dx.doi.org/10.1126/science.2169648
>
> If anyone knows of earlier cases, I'd like to hear about it!
>
> -James Holton
> MAD Scientist
>
> On 3/13/2013 7:38 AM, Alan Cheung wrote:
>> Hi all - i'm sure this many will know this : when and what was the first protein structure solved on a synchrotron?
>>
>> Thanks in advance
>> Alan
>>
>>
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