Hi Mark,
BLR(DE3) should be what you are after.
http://www.emdmillipore.com/life-science-research/blrde3-competent-cells/EMD_BIO-69053/p_yYub.s1Ol18AAAEjORl9.zLX
hth, Marko
On Wed, 27 Mar 2013, Mark J van Raaij wrote:
> Dear All,
> we were wondering if knows of an Escherichia coli B strain that is Rec deficient (Rec-).
> We want to compare certain properties of E coli B and K12, and for the experiment it would be best to use a Rec- variant.
> We have TOP10, which is a K12 derivative that is Rec-, but derivatives of B like BL21 are Rec+.
> Have a happy Easter,
> Mark
>
> Mark J van Raaij
> Lab 20B
> Dpto de Estructura de Macromoleculas
> Centro Nacional de Biotecnologia - CSIC
> c/Darwin 3
> E-28049 Madrid, Spain
> tel. (+34) 91 585 4616
> http://www.cnb.csic.es/~mjvanraaij
>
_____________________________________
Marko Hyvonen
Department of Biochemistry, University of Cambridge
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http://www-cryst.bioc.cam.ac.uk/groups/hyvonen
tel: +44-(0)1223-766 044
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fax: +44-(0)1223-766 002
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