If SPG buffer is what I think it is, that means you have a significant
concentration of inorganic phosphate, which forms salt crystals when
mixed with divalent metal ions.
-Nat
On Thu, Feb 7, 2013 at 2:24 PM, amro selem <[log in to unmask]> wrote:
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> Hallo my colleagues.
> i hope every one doing ok . i did screening since two weeks . i noticed
> today this crystals. i don`t know either it salt or protein crystal . my
> protein has zero tryptophan so i could distinguish by UV camera.
> the condition was conditions:
> 0.1M SPG buffer pH 8 and 25%PEG 1500. in addition to Nickle chlorid 1mM.
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> best regards
> Amr
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