So, I know I say this every time I post on this board, but here it goes
again.
I'm at an undergrad only school, and every 2 years I teach a class in
protein crystallography. This year I'm being super ambitious, and I'm
going to take a class of 16 to the synchrotron for data collection.
It's just an 8 hour thing, to show them the entire process. I'm hoping
that we can collect 5-6 good data sets while there.
I would like them to grow their own crystals, and go collect data. Then
we'd come back and actually do a molecular replacement (pretty
easy/standard really). Just to get a feel for how it works.
The protein I do research on is not one that I would push on this, as
the crystals are hard to grow, they are very soft, and the data just
isn't the best (resolution issues). I do have a few that will work on
my proteins, but I was thinking of having others in the class grow up
classic proteins for data collection. Obviously lysozyme is one, but I
was wondering what other standard bulletproof conditions are out there.
Can you all suggest some protein crystallization conditions (along with
cryo conditions) for some commercially available proteins? I'm looking
to get 6-8 different ones (and we'll just take them and see how it
goes). I wouldn't mind knowing unit cell parameters as well (just a
citation works, I can have them figure it out). I have about 7 weeks to
get everything grown and frozen and ready to go.
Any help would be greatly appreciated. It always amazes me how helpful
this group is. Thank you very much.
Dave
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