> Along these lines, what reagents do people use to promote disuflide bonds,
> i.e., the "anti-DTT?"
Glutathione (red) + Glutathione (ox), redox potential is adjusted by
varying the ratio.
Best,
Clemens
>
> JPK
>
> On Thu, Feb 28, 2013 at 2:06 AM, David Briggs
> <[log in to unmask]>wrote:
>
>> You might want to try "Disulfide by design"
>>
>> http://cptweb.cpt.wayne.edu/DbD2/
>>
>> Cheers
>>
>> Dave
>> On Feb 28, 2013 6:55 AM, "Careina Edgooms" <[log in to unmask]>
>> wrote:
>>
>>> Dear CCP4 members
>>>
>>> I wish to engineer a disulfide bond at the dimer interface of a protein I
>>> am working with. Does anyone know of any available software to assist with
>>> this?
>>>
>>> Best
>>> Careina
>>>
>>
>
>
> --
> *******************************************
> Jacob Pearson Keller, PhD
> Postdoctoral Associate
> HHMI Janelia Farms Research Campus
> email: [log in to unmask]
> *******************************************
>
--------------------------------------------------
Dr. Clemens Grimm
Institut für Biochemie
Biozentrum der Universität Würzburg
Am Hubland
D-97074 Würzburg
Germany
e-mail: [log in to unmask]
phone : +49 0931 31 84031
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