Dear Enrico,
Thank you very much for the comments, will definitely try it later.
Best,
Joy
-----Original Message-----
From: Enrico Stura [mailto:[log in to unmask]]
Sent: 2013Äê1ÔÂ7ÈÕ 10:16
To: [log in to unmask]; Joy
Subject: Re: [ccp4bb] Ammonium phosphate as cryoprotectant
Joy,
Try 80% saturated Lithium sulfate (2.5M) instead.
Just transfer the crystals and if it does not work let me have more precise
crystallization conditions and I will post you other options.
Enrico.
On Thu, 03 Jan 2013 18:33:05 +0100, Joy <[log in to unmask]> wrote:
> Dear All,
>
>
> May I have your expert opinion on the following question: what is the
> minimum concentration of ammonium dibasic phosphate if I would like to
> use it as cryoprotectant?
>
>
> Happy New Year!
>
>
> Joy
>
--
Enrico A. Stura D.Phil. (Oxon) , Tel: 33 (0)1 69 08 4302 Office
Room 19, Bat.152, Tel: 33 (0)1 69 08 9449 Lab
LTMB, SIMOPRO, IBiTec-S, CE Saclay, 91191 Gif-sur-Yvette, FRANCE
http://www-dsv.cea.fr/en/institutes/institute-of-biology-and-technology-sacl
ay-ibitec-s/unites-de-recherche/department-of-molecular-engineering-of-prote
ins-simopro/molecular-toxinology-and-biotechnology-laboratory-ltmb/crystallo
genesis-e.-stura
http://www.chem.gla.ac.uk/protein/mirror/stura/index2.html
e-mail: [log in to unmask] Fax: 33 (0)1 69 08 90 71
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