Hi Partha,
as far as I know the HC1 is available at the CLS and soon at the APS
and ALS, best wishes, Matthew.
On 2013-01-15 22:27, Parthasarathy Sampathkumar wrote:
> Dear Dr. Bowler,
>
> Which beamline in USA has this Humidity Control Device?
>
> Best Wishes,
> Partha
>
> On Tue, Jan 15, 2013 at 11:02 AM, Matthew BOWLER <[log in to unmask]
> [10]> wrote:
>
>> Hi Judith,
>> a very effective method is the use of a humidity control device -
>> this was actually the reason we developed the equations that predict
>> the RH in equilibrium with precipitant solutions. It has the great
>> advantage that you can characterize changes that occur and also move
>> straight to data collection. There are several HC1 devices
>> (developed here at the EMBL) in Europe and at least 1 in the USA -
>> there is also the FMS. Below are sole links that might help, best
>> wishes, Matt.
>>
>> Website for experiments:
>>
>
> http://www.esrf.eu/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-2/HC1b
>> [1]
>>
>> Calculation server for mother liquor RH equilibria:
>> http://go.esrf.eu/RH [2]
>>
>> Paper describing above:
>> http://scripts.iucr.org/cgi-bin/paper?S1744309111054029 [3]
>>
>> Papers describing device and methods:
>> http://journals.iucr.org/d/issues/2009/12/00/gm5010/index.html [4]
>> and
>> http://www.sciencedirect.com/science/article/pii/S1047847711000499
>> [5]
>>
>> Interface in MXCuBE that allows the design of dehydration
>> gradients, data collection and analysis of the images
>>
>
> http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/workflows/dehydration-workflow
>> [6].
>>
>> On 2013-01-15 15:46, Judith A Ronau wrote:
>>
>>> Greetings!
>>>
>>> I have recently been attempting crystal dehydration experiments
>>> to
>>> improve diffraction following the procedures from the ERSF in
>>> which
>>> crystals are exposed to increased concentrations of precipitant.
>>> I
>>> would like to know if anyone knew of any alternative methods for
>>> dehydration of protein crystals. Thanks!
>>>
>>> Best,
>>> Judith
>>
>> --
>> Matthew Bowler
>> Synchrotron Science Group
>> European Molecular Biology Laboratory
>> BP 181, 6 rue Jules Horowitz
>> 38042 Grenoble Cedex 9
>> France
>> ===================================================
>> Tel: +33 (0) 4.76.20.76.37 [7]
>> Fax: +33 (0) 4.76.88.29.04 [8]
>>
>> http://www.embl.fr/ [9]
>> ===================================================
>
>
>
> Links:
> ------
> [1]
>
> http://www.esrf.eu/UsersAndScience/Experiments/MX/About_our_beamlines/ID14-2/HC1b
> [2] http://go.esrf.eu/RH
> [3] http://scripts.iucr.org/cgi-bin/paper?S1744309111054029
> [4] http://journals.iucr.org/d/issues/2009/12/00/gm5010/index.html
> [5]
> http://www.sciencedirect.com/science/article/pii/S1047847711000499
> [6]
>
> http://www.esrf.eu/UsersAndScience/Experiments/MX/How_to_use_our_beamlines/workflows/dehydration-workflow
> [7] http://embl.fr/tel:%2B33%20%280%29%204.76.20.76.37
> [8] http://embl.fr/tel:%2B33%20%280%29%204.76.88.29.04
> [9] http://www.embl.fr/
> [10] mailto:[log in to unmask]
--
Matthew Bowler
Synchrotron Science Group
European Molecular Biology Laboratory
BP 181, 6 rue Jules Horowitz
38042 Grenoble Cedex 9
France
===================================================
Tel: +33 (0) 4.76.20.76.37
Fax: +33 (0) 4.76.88.29.04
http://www.embl.fr/
===================================================
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