Dear Yuri,
I am a big fan of paratone oil (or similar oils) for freezing the "holy"
crystal. The only drawback is that after you have frozen it in the oil its
a little bit hard to recover the crystal if you want to seed from it, as
its somewhat sticky...
Best of luck!
Marc
Dr. Marc Kvansakul
Laboratory Head, NHMRC CDA Fellow
Dept. of Biochemistry| La Trobe University | Bundoora
Rm 218, Phys Sci Bld 4, Kingsbury Drive, Melbourne, 3086, Australia
T: 03 9479 2263 | F: 03 9479 1266 | E: [log in to unmask] |
On 1/12/12 8:22 AM, "Yuri Pompeu" <[log in to unmask]> wrote:
>Dear community,
>I have what seems to be a pretty decent single crystal that grew from a
>screen set up 2 weeks ago.
>I am trying to reproduce it but so far I have not succeeded. I am however
>afraid the crystal that did form will start to deteriorate. So this
>brings me to dilemma, I feel like I should try and mount this crystal and
>shoot it. But since I only have 1 sample, I do not want to mess this
>up... I am inclined to try cryo conditions, but I am afraid the addition
>of a cryo such as glycerol could destroy the little guy.
>The crystal formed in 30% PEG 4000, 0.1M NaCitrate pH5.6 and 0.2M NH4AcO,
>I wonder if this is a cryo condition already?
>Any suggestions would be appreciated.
>
>best,
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