Ulrike,
I usually suggest it as the second try (the first try is mother liquor
alone), as it does not involve mixing any new buffer concoctions. I do
not have hard data, but I'd estimate it worked in about 50% of cases; it
seemed not to matter if you try it on a soluble or membrane protein
crystal. In my hands it performs better than mineral oil, silicon oil or
paratone. Problematic cases are crystals in heavy precipitate, which has
to be removed prior to transfer into the oil, otherwise it sticks around
the crystal and you can't get the crystal "dry".
hth,
Jens
On Mon, 2012-12-03 at 13:07 +0000, Ulrike Demmer wrote:
> Dear crystallographers,
>
> does anyone have experience using Perfluoropolyether (Hampton Research) as cryoprotectant for membrane proteins ?
>
> Cheers,
>
> Ulrike
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