Dear All,
I have read a recent paper by Salgado et al about using non-auxotrophic E.coli to incorporate SeCys into recombinant protein for phasing purposes. Does anyone have a source for Selenocysteine? I have also seen a paper by Schaefer et al which uses nitric acid treated elemental Se for a sulfur surrogate to generate Se-labeled protein. Has anyone else tried this? My proteins are rich in Cys and tend to lack Met so the prospect of labeling cys is very attractive.
Thanks,
Linda
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Linda Olson, PhD
Research Scientist II
Dept Biochemistry
Medical College of Wisconsin
8701 Watertown Plank Rd
Milwaukee, WI 53226
phone: 414-955-8545
fax: 414-456-6510
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