Maybe low-spin, ferric cytochrome? 420 would be the soret peak
and 520 the alpha+beta peaks which are very broad in the
oxidized form. Add a little dithionite or ascorbate and see if
the soret peak shifts to longer wavelength and the alpha peak
sharpens up at 550-560 nm
Grishin, Andrey wrote:
> Dear CCP4bb users,
> this problem was already discussed many times here and I've read these
> discussions before writing. I have a pale yellow protein. The spectrum
> contains peaks at 320 and 420. Before we proceed to ICP-OES and
> wavelength scans at the synchrotron, may be some of you had a similar
> spectrum before? Does not look like FAD, NADH and pyridoxal phosphate.
> Thank you
> Andrey Grishin
> Postdoctoral Fellow
> University of Saskatchewan
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