Reply-To: | | [log in to unmask][log in to unmask], 9 Nov 2012 10:09:07 -0700621_us-ascii I meant "where the drops have the concentration of ammonium acetate needed".
James
On Nov 9, 2012, at 10:06 AM, James Stroud wrote:
> This sounds like a job for ammonium acetate. Use it as your salt. Purify your complex in it and then set up drops where they wells have the amount of ammonium acetate needed to keep your protein stable and the wells have none, or a range of concentrations. The ammonium acetate will equilibrate by vapor diffusion, lowering the concentration in the drop and causing your complex to come out of solution. > > James > > [...][log in to unmask] |