How about DNA synthesis within crystals?
1: Kiefer JR, Mao C, Braman JC, Beese LS. Visualizing DNA replication in
a catalytically active Bacillus DNA polymerase crystal. Nature. 1998 Jan
15;391(6664):304-7. PubMed PMID: 9440698.
2: Johnson SJ, Taylor JS, Beese LS. Processive DNA synthesis observed in
a polymerase crystal suggests a mechanism for the prevention of
frameshift mutations. Proc Natl Acad Sci U S A. 2003 Apr
1;100(7):3895-900. Epub 2003 Mar 20. PubMed PMID: 12649320; PubMed
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Meindert
On 10/9/12 2:33 PM, WENHE ZHONG wrote:
> Dear CCP4 members,
>
> Recently, I got a ligand soaking structure to clearly show a large
> domain (~100 amino acids) movement compared to the no soaking structure.
> Although there are some reported examples of this enzyme to suggest the
> flexibility of this large domain which is relevant to substrate binding.
> _But it is the first time I can see it happen in crystal soaking
> procedure._ In this case, I am pleased by this result.
>
> My question is, do you have any other example like mine, where domain
> (or loop) movement is observed _*in crystal*_ during ligand _*soaking*_
> procedure? It would be very helpful for me to relate my result to other
> similar examples. Thank you very much.
>
> King regards,
> Wenhe
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Meindert H. Lamers
Medical Research Council
Laboratory of Molecular Biology
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