Dear Karolina,
We had one nice example where the protein could easily be concentrated to about 50mg/mL without crystallising, at which point we realised that it would be a good project for collaborating with our NMR colleagues, i.e. the RING domain of the Kaposi's sarcoma-associated herpesvirus K3 protein (Dodd et al., JBC 279:53840-53847, 2004). I wonder if you'd find other examples by looking at NMR structures -- unless, of course, you're after proteins of still unknown structure!
Best wishes,
Randy
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Randy J. Read
Department of Haematology, University of Cambridge
Cambridge Institute for Medical Research Tel: +44 1223 336500
Wellcome Trust/MRC Building Fax: +44 1223 336827
Hills Road E-mail: [log in to unmask]
Cambridge CB2 0XY, U.K. www-structmed.cimr.cam.ac.uk
On 24 Oct 2012, at 22:47, Karolina Michalska wrote:
> Hello everybody,
>
> I'm looking for small proteins (up to 150 aa) that are known to express
> reasonably in E. coli, are soluble and folded but failed to crystallize.
> Can you provide some examples, especially of proteins that might be
> interesting for a broad community?
>
> Thanks,
> Karolina
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