I also recommend you to check Dan's link on the paper for stabilising
protein solubility.
I have similar experience. I got high yield, soluble protein but
aggregates once I have purified concentrated protein.
Before venturing to the detergent area, I would suggest using other
additives first (kosmotropes, chaotropes, amino acid, sugars and
alcohols). I managed to get it work in high NaCl, KCl and NaBr.
I managed to purify the protein, put it in Tris buffer with low NaCl.
I concentrated the protein and work fast enough before it crashes (my
protein usually crashes overnight). Then aliquot out the protein in
different eppendorf tubes containing different additives, leave it
overnight; the next day, I spun all the tubes and check for
aggregation (of course, make sure you leave a "control", which in my
case is Tris buffer, low NaCl; just for a point of comparison). I got
7 tubes then which do not have aggregated pellet; I left them for few
more days; 4 of them aggregated, and 3 of them looks okay.
Allan
Quoting Vitali Stanevich <[log in to unmask]>:
> Hi,
>
> Sorry for off-topic question.
>
> Does anyone have experience of the stabilisation of water-soluble proteins
> by detergents? Protein I'm working with is definitely water-soluble and has
> high yield, but, unfortunately, not very stable. Especially during
> concentration. So, we thought that adding some detergents may one of the
> ways to stabilise protein.
>
> So, did anyone do it before or may be know published examples? Any
> suggestions on the detergent type/concentration would be welcome.
>
> Thanks,
> Vitali
>
--
Allan Pang
Visiting Scientist
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Queen Mary University of London
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