Err, so the HNCACB has positive and negative peaks ... So why don't you tell Analysis to pick positive *and* negative peaks?
Markus
-----Original Message-----
From: CcpNmr software mailing list [mailto:[log in to unmask]] On Behalf Of Justin Lecher
Sent: Tuesday, September 11, 2012 10:26 AM
To: [log in to unmask]
Subject: Re: Add CO/HA/CA/CB only work for CB??
On 11/09/12 18:58, Justin Lecher wrote:
> Hello,
>
> doing a standard seq. assignment with an HNcoCACB as query and an HNCACB
> as match. Both spectra have root dims assigned. If I now do a sequential
> link, the non root resonance gets created as expected. But when I click
> "Add...." only the CB is set, but the resonance which should be CA is
> removed again. Was this observed before?
>
> Thanks,
> Justin
>
Okay,
I found something "bad".
If I set "pick only positive peaks", the CB are marked as CB and the CA
are omited. In contrast, setting "pick negative only" brings the CB
assignement to the CA and the CB are omited this time. This is wrong.
Picking both, lets all peaks be unassigned.
If I compare my spectra with the tutorial ones, the difference in the
signs of the peaks e.g. CB(tutorial) = -1 * CB(justin).
Is this fixable?
Justin
--
Justin Lecher
Institute of Complex Systems
ICS-6 Structural Biochemistry
Research Centre Juelich
52425 Juelich, Germany
phone: +49 2461 61 2117
|