Dear Tanya, We have published a paper related to this topics on PloS One last year (see below), and we have not generally encountered "crystal degradation pattern upon X-ray exposure".
"Get phases from arsenic anomalous scattering: de novo SAD phasing of two protein structures crystallized in cacodylate buffer."
Liu X, Zhang H, Wang XJ, Li LF, Su XD.
PLoS One. 2011;6(9):e24227. Epub 2011 Sep 2.
Xiao-Dong Su, Professor
Chairman of the Commission on Biological Macromolecules (CBM),
International Union of Crystallography (IUCr);
Secretary-general, Chinese Crystallographic Society(CCrS);
School of Life Sciences, Peking University
100871 Beijing, China
Phone: +86-10-62759743
FAX: +86-10-62765669
E-mail: [log in to unmask]
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发件人: Tatyana Sysoeva <[log in to unmask]>
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已发送邮件: Mon, 30 Jul 2012 06:53:13 +0800 (CST)
主题: [ccp4bb] asking for a reference for cacodylate decomposition in protein crystals upon X-ray exposure
Hi!
I heard a couple of times that use of cacodylate buffers in crystallization
is bad, and not only because of the compound toxicity.
As I understood, presence of the cacodylate in a protein crystal will cause
a particular crystal degradation pattern upon X-ray exposure - "darkening
of the crystals, gas formation"
I tried to find some references on that and failed in doing so.
I found some earlier discussions like this one:
http://www.proteincrystallography.org/ccp4bb/message23691.html
but don't have anything to reference in literature. I would appreciate if
someone can point me to a right direction.
I am sorry if this question is out of the groups topic range.
Thank you in advance!
Sincerely,
Tanya
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