On Wed, Aug 1, 2012 at 11:27 AM, Uma Ratu <[log in to unmask]> wrote:
> The protein is in tetramer form. I define this by using the residue number
> (1332) which is 4 x monomer.
>
> After run, Phaser only gave 9 partial solutions, and no solution with all
> components. The resulted PDB contains only dimer form of the protein, not
> the tetramer. And the first TFZ score is around 2.5, which is too low for
> MR.
>
> I have the report file of data processing and the summary of Phaser
> attached.
>
> Could you please advice which part is wrong, why can I get the tetramer form
> of the protein?
Your data are processed as P2, which is much less common (for
proteins) than P21, and it looks like you haven't told Phaser to try
P21 too. There are many other reasons why MR might not work, but I
think it's very likely that the space group is wrong.
-Nat
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