Reply-To: | | [log in to unmask][log in to unmask]> a écrit:
Hello Rashi There is no problem with ITC at "low temperature", apart for a likely slower binding and, hence, lower heat power signal. Do not conclude that there is no binding if deltaH is close to 0 around 20-25 °C. You may well have DeltaH = 0 at a given temperature, and yet the affinity constant is maximum at that temperature (by the Van't Hoff equation). However, if you have no signal (DeltaH = 0) at several temperatures, this means that this peptide does not bind. It is [...]34_6a1-50237c80-5f-5cb80180@2120484540_0_ |