Hello,
On the Initialise Root Resonances issue, did you click the "Update Table"
button at the bottom right (after clicking Initialise Peak List)? That
should re-calculate possible NH2 pairs.
Now since you are able to see the HSQC in the Initialise Root Resonances
dialog that means you must have set the experiment type to 15N HSQC/HMQC,
correct? If so I cannot see why it is not being shown in the Root Peak
List of the Pick and Assign from Roots dialog, it ought to be. I just
opened up an HSQC (in a blank project) and it worked ok for me. Is there
any error message in the console? If none of this sorts this out can you
send me the project *off list*.
Wayne
On Mon, 16 Jul 2012, Kyle wrote:
> Greetings,
>
> I have two issues on which I would like some advice:
>
> 1). I know my protein has multiple side chain amines, I can guess what
> they are in the HSQC. However, I'm curious why when I open "Initialize
> Root Resonances" popup window, the Sidechain Amine table is not
> populated. Is there a step I'm missing? The 15N resonances are well
> within the specified tolerances (0.02 ppm)
>
> 2) after I initialize the root resonances, I want to propagate the
> assignment through a number of standard backbone experiments, e.g. HNCO,
> HNCA, HNCOCA, etc. When I open the "Pick and Assign from Roots" window,
> I try to select my root peak list and root peak window, but the only
> options are <NONE>. Why is my HSQC window and associated peak list not
> recognized?
>
> Any pointers would be most appreciated.
>
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