Hi Jan,
you will find some methods for detection of thiols and/or disulfides there:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Thiols_and_disulfides
HTH,
Guenter
> Dear all,
>
> I am working on a protein where I have to stabilize the closed
> conformation of the protein using disulphide bond. The strategy to
> design the cysteine mutants is based on the molecular dynamic
> simulations, and accordingly the residues were chosen. The ultimate
> goal is to trap the ligand in closed conformation of protein and
> crystallize it. I am facing few issues: Is there some reliable assay
> that can check the formation of disulphide bonds in protein.
> Additionally, does anybody knows another method(s) that can be used to
> trap a closed conformation. I look forward for your suggestions and
> discussions on this issue.
>
> Thanks very much!
>
> Jan
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