Dear All,
Thanks alot for your valuable suggestion.I hope I will find out the
solution now. As far as to giveup is out of question!!!!
Thanks once agin
Regards;
Bashir
On Wed, July 11, 2012 05:25, Tuhin Bhowmick wrote:
> Dear Muhammad,
>
> I had a similar case, and the crystals could indeed be optimized. A few
> things to check first,
>
> 1) How long does it take for the needles to appear? Sometimes, if the
> protein is degraded/ cleaved over time, a small population (possibly a
> fragment of the whole
> protein) from the heterogeneous mix can give similar crystals. Like
> Bryan had suggested, it is also very useful to check the mol wt. of the
> crystallized species through
> mass spect/ native page. But do make sure to give the crystals serial
> washes, so the test accounts for crystallizexd species, not the ones from
> surrounding condition.
>
> 2) If the crystals indeed contain the protein of interest, they can be
> used
> for various seeding methods. I've got results from both streak and micro
> seeding.
>
> Best,
>
> Tuhin.
>
> Tuhin Bhowmick
>
> Department of Physics
>
> Indian Institute of Science
>
> Bangalore: 560012
> Email: [log in to unmask]
>
> On Wed, Jul 11, 2012 at 12:34 AM, Bernhard Rupp (Hofkristallrat a.D.) <
> [log in to unmask]> wrote:
>
>> > Always give up.****
>>
>> ** **
>>
>> ...definitely not the kind of guy I want to sit up front in an
>> airliner…**
>> **
>>
>> ** **
>>
>> Best regards, BR****
>>
>> -----------------------------------------------------------------****
>>
>> Bernhard Rupp, ATP-B737, CFII-MEI****
>>
>> Vienna Air International****
>>
>> Professional Aviation Services****
>>
>> 001 (925) 209-7429****
>>
>> +43 (676) 571-0536****
>>
>> [log in to unmask]****
>>
>> [log in to unmask]****
>>
>> http://www.vienna-air.com/ ****
>>
>> -----------------------------------------------------------------****
>>
>> It is not your aptitude but your attitude****
>>
>> that determines your altitude. (or your crystals)****
>>
>> -----------------------------------------------------------------****
>>
>> ** **
>>
>> ** **
>>
>> ** **
>>
>> From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
>> yybbll****
>>
>> Sent: Tuesday, July 10, 2012 11:58 AM****
>>
>> To: [log in to unmask]****
>>
>> Subject: Re: [ccp4bb] Crystal Optimization****
>>
>> ** **
>>
>> Hi, ****
>>
>> ****
>>
>> In my experience, it is very very very difficult to optimize this needle
>> like crystal. Always give up.****
>>
>> ****
>>
>> Good luck!****
>>
>> ****
>>
>> ****
>>
>> Dear All;****
>>
>> Could somebody give a nice suggestion how the following type crystal
>> could
>> ****
>>
>> be optimized, I almost tried everything.****
>>
>> Crystal Image is attached****
>>
>> Crystal condition: 20% w/v PEG3350 and 200mM NaCl.****
>>
>> Thanks in advance****
>>
>> Bashir****
>>
>> -- ****
>>
>> Muhammad Bashir Khan****
>>
>> ******************************************************
>>
>> Structural Genome Consortium (SGC). University of Toronto****
>>
>> Toronto, Canada****
>>
>
--
Muhammad Bashir Khan
**************************************************
Department for Structural and Computational Biology
Max F. Perutz Laboratories
University of Vienna
Campus Vienna Biocenter 5
A-1030 Vienna
Austria
Austria
Phone: +43(1)427752224
Fax: +43(1)42779522
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