Hi Leonard,
I would treat this like a MS/MS ion search experiment looking for the peptide via MASCOT or similar. Talk to some local Mass Spec people, I'm certain they could identify it and then you'd be closer to an explanation.
John.
Dr. John Hinks
Syngenta
Jealott's Hill International Research Centre
Bracknell
Berkshire
RG42 6EY
United Kingdom
[log in to unmask]
www.syngenta.com
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Leonard Thomas
Sent: 18 July 2012 23:12
To: [log in to unmask]
Subject: [ccp4bb] peptide fragment
Hello All,
I have been working on a structure for one of the research groups here
and have come across a peptide fragment of about 10 residues. The
protein forms a tetramer and the peptide is found between two of the
monomers. The protein itself does not require a peptide for function
or formation for that matter. Has anyone come across similar things?
and have a reference. We are trying to figure out where it may have
come from. My best guess so far is from somewhere in the purification
though I have no idea how long the protein sat around before
crystallization.
Cheers,
Leonard Thomas Ph.D.
Macromolecular Crystallography Laboratory Manager
University of Oklahoma
Department of Chemistry and Biochemistry
Stephenson Life Sciences Research Center
101 Stephenson Parkway
Norman, OK 73019-5251
[log in to unmask]
http://barlywine.chem.ou.edu
Office: (405)325-1126
Lab: (405)325-7571
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