On Mon, 25 Jun 2012, Pernille Møller wrote:
> How can I figure out to "set appropriately" to pick the peaks where they belong??
> For my NH-HSQC spectrum I have:
>
> dim2 (15N); minimum aliased frequency (ppm): 95.602, maximum aliased frequency (ppm):
> 135.602
>
> For my HNCA spectrum I have:
>
> dim2 (15N); minimum aliased frequency (ppm): 108.001, maximum aliased frequency (ppm):
> 130.001
>
> Well, I don’t know how to set these parameters so that I get the actual N-shift (127.50)
> for my aliased peak?
Set the 15N min & max for the HNCA to be the same as for the HSQC and all
will be well!
As a general rule, it's a good idea to always record two 15N HSQCs with
different sweep widths so you can work out which peaks are aliased (and by
how much) even if you don't use 90, -180 phase corrections to allow you to
tell by the sign of the peak. Remember that you may also have Arg NE
sidechain resonances (~80 ppm) and even His sidechain resonances in there,
so look out for them. Once you have established the shift range for your
aliased peaks in the HSQCs, then apply the same min & max to all your
triple resonance expts.
Dr. Brian O. Smith --------------------------- Brian Smith at glasgow ac uk
Institute of Molecular, Cell and Systems Biology & School of Life Sciences,
College of Medical, Veterinary & Life Sciences,
Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK.
Tel: 0141 330 5167/6459/3089 Fax: 0141 330 4600
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