Add DNA?
Also, polydispersity is not great for crystallization but may not be
the end of the world :)
Artem
On Mon, Jun 18, 2012 at 4:52 AM, Arpit Mishra <[log in to unmask]> wrote:
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> Dear CCP4BB members
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> Sorry for off topic, i had question related to dispersity of proteins of my
> interest, i am working on DNA binding protein, having PI around 9 to
> 10,Initially protein was remaining stable at high salt, but was not able to
> concentrate more than 1mg/ml, after using 50mm Arg, 50mm Glutamate i
> overcome the solubility and concentrating problem but problem now is that
> my protein is remaining polydisperse, so i want to try different
> additives, i had tried some detergents and chaotropic agent,pH ranges.but
> it didnt help, so any suggestions to bring my protein in monodisperse state
> that will be really helpful...thanks for your suggestions in advance
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> Arpit Mishra
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