Google yields amongst others:
His-tag impact on structure
Acta Cryst. (2007). D63, 295–301
...quäl dich.
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of weliu
Sent: Tuesday, June 26, 2012 6:07 PM
To: [log in to unmask]
Subject: [ccp4bb] The effect of His-tag location on crystallization
Dear all,
We crystallized a protein and found that crystal quality greatly depended on
the location of His-tag. When a His-tag was added at the C-terminus, only
crystalline precipitate or spherical quasi crystals were grown. However,
when the His-tag was moved to the N-terminus, single crystals were grown
under a number of conditions, and the best one diffracted to 1.7 angstrom
after optimization. I was wondering if there were published reports
describing similar cases.
Thank you in advance
Wei Liu
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