While a comparison of the B factors is easy to do, interpreting its meaning
is not. The B factors are affected not only by the motion of the atoms, but
by many other factors, including the overall quality of the order of the crystal.
The B factors for a model based on a low resolution data set will be larger than
those from a model of the exact same protein based on a high resolution data set.
If you wish to derive some sort of structural interpretation to the B factors
of your region of interest you have to somehow normalize them relative to the
average B factor of the better ordered parts, or, say, the Wilson B.
Dale Tronrud
On 05/11/12 02:32, <World light> wrote:
> Hi,
>
> I would like to make the comparative study of different mutant structures. There
> is a region which is flexible and missing in some cases. Is there any direct
> method of calculating the BAVERAGE of that region. I used the baverage program
> in ccp4 for whole structure but would like to see the values with and without
> the region.
>
> Thanx,
>
> Bishwa
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