Hi Jose,
>Has anyone worked with, or seen an example of, a solvent accessible disulfide that cannot be reduced with TCEP?
Something to keep in mind is that TCEP is not very stable with phosphate buffers near neutral pH.
>Alternatively solvent accessible disulfides that resist reduction with DTT but can be reduced with TCEP?
This could be due to the effective pH of TCEP HCl (~1.5-8.5) being wider than DTT ~7.
Take Care,
Sean Seaver
P212121
http://store.p212121.com/
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