If you express the protein as an intein fusion you can make a C-terminal thioester, which you can then modify quite specifically with various reagents .
Pat
On 25 May 2012, at 10:00 AM, Sebastiano Pasqualato wrote:
>
> Dear all,
> is anyone aware of a way to selectively protect the carboxylate at a protein C-terminus, while leaving unaffected those of Asp and Glu side chains?
> Thanks a lot in advance,
> have a nice weekend,
> ciao,
> s
>
>
> --
> Sebastiano Pasqualato, PhD
> Crystallography Unit
> Department of Experimental Oncology
> European Institute of Oncology
> IFOM-IEO Campus
> via Adamello, 16
> 20139 - Milano
> Italy
>
> tel +39 02 9437 5167
> fax +39 02 9437 5990
>
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