My fellow crystallographers,
We are thinking the SUMO/His vectors would be nice to have in the lab
aresenal... but. The stumbling block is that the protease needed for
cleavage is very expensive at crystallography scale. SUMO(ULP-1)
protease costs ~$700/mg fusion protein. It would not be a problem
for labs using micrograms of protein, but is prohibitive at our level
of protein purification.
Has anyone found a way around this? Your pal, Gloria
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