I have used both Novagen thrombin & lyophilized thrombin of BDBioscience(dissolved in 10X thrombin dilution buffer provided by Novagen) in both 'thrombin cleavge buffer' & others (HEPES pH 7.0, Bicine pH 9.0, MOPS pH 7.8) without adding calcium chloride - all the conditions permitted the cleavge process, but addition of DTT/ BMe slowed the process
|