Hi all,
I am working on an oxidoreductase and having some trouble during molecular replacement.
The resolution of the crystal is 1.7 A and the space group is I4122 (a = b = 121.086, c =156.93 and alpha = beta = gamma = 90).
The cell content analysis results predicted two molecules in the asymmetric unit and the solvent content as 36 %. The Matthew's coefficient is 1.94. The Wilson plot also looks fine.
However, after molecular replacement (using Phaser) the result just gives me just a single molecule. ( RFZ = 8.5 TFZ =13.9 PAK = 0 LLG =189 TFZ=18.0 LLG = 642)
Any suggestions how to solve this problem?
Thanks in advance.
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