Reply-To: | | [log in to unmask][log in to unmask], 3 Apr 2012 12:06:38 -0400532_WINDOWS-1252 Dear Toby,
I don't think there is a basic problem using glycerol in crystallization. Glycerol will affect the vapour pressure (if it is not present in the well/precipitant solution) and 10 % glycerol is ~ 1.3 molar concentration. During equilibration the drops may increase in volume, decreasing the protein concentration. Thus, when using glycerol I think it is generally beneficial to start with a high protein concentration. Perhaps, you can concentrate your protein sample further. [...][log in to unmask] |