I have a titration with 11 total 1H-15N HSQC spectra. I've got the spectra
imported/overlaid in Analysis 2.1.5, updated last week. Spectra were
converted with pipe2azara, and I've made contour files. All are peak
picked, and I'm trying to propagate assignments prior to extracting Kd
with "follow shift changes". This is a "clean" project with no other
spectra in it.
Now the problem: any time I zoom, move, or otherwise change the display,
all 11 spectra and associated peak markers draw/update pretty much as
expected. As soon as they finish drawing, or perhaps as soon as I so much
a breathe on my mouse, everything on the canvas blanks. If I remember
where things are, I can blindly draw a box around a shifting resonance,
which redraws and re-blanks, and propagate the assignments. For the
peripheral peaks, this is very annoying, but I have a handful of places
where there is significant overlap and do need to see what I'm doing.
I tried zooming in on only 2 or 3 peaks, but that only slightly speeds up
the display problem. If I turn off the display for several spectra, things
seem to behave normally - I'm not sure what the magic number is, but it's
less than 10.
Any suggestions? I'd prefer not to have to propagate every assignment 2 or
more times. I've allocated a full gig of RAM to Analysis on startup, so I
shouldn't be hitting bit problems there.
I've seen similar things happen temporarily with some large 3D spectra
(C13-NOESY/HCCH-TOCSY), but those are usually ok after a redraw. Quitting
and restarting makes no difference.
Cheers,
Andrew
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