Dear Nicolas,
Pleased to hear it all got solved.
If the error was general I suspect that you got the wrong experiment type
for the NOESY, or the wrong dimension mapping, which amounts to the same
thing. That is not an error of yours - you can have either HSQC-NOESY or
NOESY-HSQC, the wrong choice gives this kind of problems, and it is quite
tricky to get right. If the error was limited to CH(CH3)2 groups it must
have been some assignment error. This kind of thing could be solved,
either by you or by me, but if we are already there I guess there is no
need.
Cheers,
Rasmus
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Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Mon, 26 Mar 2012, Nicolas Jean wrote:
> Dear Rasmus H. Fogh,
>
> I finally solved both problems :
>
> About my Leucines : I tried to deassign my Cδs and Hδs using the
> "Deassign" button at the bottom of the "Resonance" chart ( Resonance >
> Resonances) (instead of doing with the Assignment Panel as I did before)
> and accepting to remove the resonance assignments to my peaks. I then
> re-assigned these resonances, only on my HSQCs and this time the
> assignments were ok.
>
> About my red "bound" boxes in the Quality report chart : In order to zip
> up my project to you, I deleted my Noesy. Then I noticed that all these
> boxes in the Quality Report chart went back to normal. After a few
> checks, it seems that there was an unknown problem with my attribution
> in these spectra, a deletion of their peaklist is enough to solve this
> issue (and the Leucine one).
>
> I must have done a wrong operation (of unknown nature) during my Noesy
> assignments. I shouldn't need them anymore for my project, the
> assignments being finished (and having them on HSQCs), so it doesn't
> matter if I delete them.
>
> Thank you a lot for your help,
>
> Nicolas
>
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