Dear Carolin,
Tim and I have both looked at the code, but nothing obvious comes to mind.
Before you do anything else, could you update your version with the newest
patches, and check that the error persists?
Assuming this does not fix things, could you send me the project to test,
and also tell me which program version you are running? As usual the
project whould be zipped up, *exlucing* spectrum files.
Thanks,
Rasmus
---------------------------------------------------------------------------
Dr. Rasmus H. Fogh Email: [log in to unmask]
Dept. of Biochemistry, University of Cambridge,
80 Tennis Court Road, Cambridge CB2 1GA, UK. FAX (01223)766002
On Tue, 6 Mar 2012, Seuring Carolin wrote:
> Dear all,
>
> Looking at the Quality Reports for the protein I work on using
> solid-state NMR data, I get the following assignment error messages for
> some residues : "Impossible bond" for a residue as ThrCa-ThrN in a NCA
> or "excessive bonds" for a LysC-LysCa-LysN assignment in a NCaCx. I am
> very confident that the Quality report is not correct, so I am wondering
> if this error appears because of one of the following reasons or
> elsewise. - assumptions CCPN makes, that are only valid in liquid state
> or - tight set reference rc shifts - or experiment types (I chose
> N_CA.onebond (through-space) for the NCA and
> N_CA_C.onebond,through-space for the NCacx)
>
>
>
> Thank you for your answer!
> Carolin
|