Dear all,
I have noticed in my CcpN project that CcpNmr (Analysis 2.2.1) doesn't
assign correctly most of my Leu Hδs with their respective Leu Cδs in my
non stereospecific assignment. For example, for a Cδ-Hδ pair (from a
given residue), the software assign the carbon "Cδa" and its respective
proton "Hδb" instead of "Hδa". So finally I have some Cδa-Hδb and
Cδb-Hδa pairs, which are confusing for the structure calculation step.
In some other cases (still for Leu residues), the software gives the
right assignment for the carbon associated to its Hδ (i.e : "Cδa" for
the one linked to "Hδa"), but also gives the same name to the other
carbon (which should be "Cδb", as it is linked to the "Hδb" for example,
instead of "Cδa"), with the result that I eventually get 2 distinct
resonances in CCPNmr called Cδa (or Cδb).
I tried to de-assign and reassign both Hδ and both Cδ, assign them in
another order, but CcpNmr always makes the same assignment choice than
before.
I have also noticed the same issues for the Valine Cγs and Hγs.
Does anyone know how to solve it? Thank you for your help.
Nicolas JEAN
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