Dear all,
Looking at the Quality Reports for the protein I work on using solid-state NMR data, I get the following assignment error messages for some residues :
"Impossible bond" for a residue as ThrCa-ThrN in a NCA or "excessive bonds" for a LysC-LysCa-LysN assignment in a NCaCx.
I am very confident that the Quality report is not correct, so I am wondering if this error appears because of one of the following reasons or elsewise.
- assumptions CCPN makes, that are only valid in liquid state or
- tight set reference rc shifts
- or experiment types (I chose N_CA.onebond (through-space) for the NCA and N_CA_C.onebond,through-space for the NCacx)
Thank you for your answer!
Carolin
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