For those who want to stay with the CCP4 GUI, yu can rn SHELXC/D/E from it
and get the advantage of keeping all info in the same place.. The Zn edge
is _ 1.28 but you can use the Zn signal for any wavelength less than that..
Eleanor
On Mar 6 2012, Bosch, Juergen wrote:
> Since you've collected the data already use your favourite data
> processing program and treat the Friedel pairs separately. I'd suggest to
> try HKL2map in conjunction with SHELX C/D/E (sorry for the non CCP4
> advertisement here) for solving the heavy atom sites. You can in parallel
> also try SnB or BnP to find a substructure solution. Depending how bad
> you resulting density looks like you might want to improve your phases
> via Sharp.
>
>If you want to stay in the CCP4 protected sandbox, then give Crank a try.
>
>Jürgen
>
>On Mar 6, 2012, at 3:24 PM, Francis E Reyes wrote:
>
>http://skuld.bmsc.washington.edu/scatter/AS_form.html
>
>Maybe useful to you.
>
> However, I would advise to do a fluorescence scan over the range given in
> the graph and then use chooch to provide the precise energies for your
> peak and inflection.
>
> If you have a large crystal don't expose all of it when you do the
> fluorescence scan but rather reserve a 'fresh' piece to do your actual
> data collection.
>
>F
>
>
>On Mar 6, 2012, at 1:09 PM, Deepthi wrote:
>
>Hi
>
> I am trying to solve the structure of an engineered protein.The protein
> is crystallized with Zn bound to it .We collected a 1.5A0 data. Molecular
> Replacement didn't yield a good match for the protein. I want to try MAD
> taking advantage of the Zn atoms in protein. I am not sure what
> wavelength should i use to collect the diffraction data for Zn. any
> suggestions?
>
>Thank You
>Deepthi
>
>--
>Deepthi
>
>......................
>Jürgen Bosch
>Johns Hopkins University
>Bloomberg School of Public Health
>Department of Biochemistry & Molecular Biology
>Johns Hopkins Malaria Research Institute
>615 North Wolfe Street, W8708
>Baltimore, MD 21205
>Office: +1-410-614-4742
>Lab: +1-410-614-4894
>Fax: +1-410-955-2926
>http://web.mac.com/bosch_lab/
>
>
>
>
>
--
Professor Eleanor Dodson
YSNL, Dept of Chemistry
University of York
Heslington YO10 5YW
tel: 00 44 1904 328259
Fax: 00 44 1904 328266
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