Which kind of buffer you use? If it is Tris, then temperature change
will cause pH change.
Actually, this is a good way for crystallization.
Kevin
On Mon, Mar 12, 2012 at 9:02 AM, Min-Kyu Cho <[log in to unmask]> wrote:
> Hi all,
>
> I have a homotetrameric coiled-coil domain sample with 45aa per each. While
> I store this sample at 4oC, the sample looks clear w/o any particles. But
> when I took out the sample to my bench at r.t, I can see there are
> precipitates (as stack of needle like particles) at the bottom of the tube
> after several hours. Interestingly, when I put it back into 4oC fridge, the
> precipitates disappeared and the solution turned into clear again.
>
> Does anyone have knowledge of such behavior of any protein? I appreciate any
> information related.
>
> Min-Kyu
|