If you are lucky (or should I say unlucky) and have an ice-ring on the same image or on another image collected during the same shift and similar distance, you can estimate the beam centre using the "fit circle" option.
Although software (and beamlines) have improved greatly, it can still be a good idea to collect ice- or wax-rings on purpose before or after data collections for determining accurate beam centres.
Mark J van Raaij
Laboratorio M-4
Dpto de Estructura de Macromoleculas
Centro Nacional de Biotecnologia - CSIC
c/Darwin 3
E-28049 Madrid, Spain
tel. (+34) 91 585 4616
http://www.cnb.csic.es/~mjvanraaij
On 22 Mar 2012, at 13:42, sonali dhindwal wrote:
> Dear All,
>
> We have collected a data for a protein crystal at SER-CAT Chicago and the detector is mar300.
> We are using mosflm to process the data.
> While indexing, the beamstop which it is taking is wrong, due to which it fails.
>
> I am trying to define the beamstop manually using tools like mask and spot search area.
> (it might be wrong)
> It will be highly appreciable if someone can please suggest if this the method we should use to define the beamstop or there is any site definition file which has to be used, as it is available for HKL2000 or how we have to define the beamstop in mosflm.
>
>
>
> --
> Sonali Dhindwal
>
> “Live as if you were to die tomorrow. Learn as if you were to live forever.”
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