Dear Jerry,
I've just become aware of this spin column to remove endotoxin, marketed by Generon, UK.
http://generon.co.uk/index.php?route=product/category&path=238
Good luck,
Jon
________________________________________
On Wed, Mar 7, 2012 at 9:50 AM, Jerry McCully
<[log in to unmask]> wrote:
>
>
> Dear All;
>
> We purified a His tag protein by Ni-NTA and gel-filtration from
> E.coli.
>
> We tried two endotoxin removal resins from Pierce. However, it is
> hard to remove the endotoxins in the purified protein because the protein
> bound to the resin as well.
>
> This protein contains quite a few aromatic residues and has a pI
> around 6.
>
> Any ideas to remove the endotoxins will be highly appreciated.
>
> best regards,
>
> Jerry McCully
>
>
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