A reference for a real MAD phasing with Zinc (worked very well):
Ennifar et al. MAD phasing replacing magnesium with zinc. Acta Cryst.
(2001). D57, 330
Philippe Dumas
"Bosch, Juergen" <[log in to unmask]> a écrit :
> Since you've collected the data already use your favourite data
> processing program and treat the Friedel pairs separately. I'd
> suggest to try HKL2map in conjunction with SHELX C/D/E (sorry for
> the non CCP4 advertisement here) for solving the heavy atom sites.
> You can in parallel also try SnB or BnP to find a substructure
> solution. Depending how bad you resulting density looks like you
> might want to improve your phases via Sharp.
>
> If you want to stay in the CCP4 protected sandbox, then give Crank a try.
>
> Jürgen
>
> On Mar 6, 2012, at 3:24 PM, Francis E Reyes wrote:
>
> http://skuld.bmsc.washington.edu/scatter/AS_form.html
>
> Maybe useful to you.
>
> However, I would advise to do a fluorescence scan over the range
> given in the graph and then use chooch to provide the precise
> energies for your peak and inflection.
>
> If you have a large crystal don't expose all of it when you do the
> fluorescence scan but rather reserve a 'fresh' piece to do your
> actual data collection.
>
> F
>
>
> On Mar 6, 2012, at 1:09 PM, Deepthi wrote:
>
> Hi
>
> I am trying to solve the structure of an engineered protein.The
> protein is crystallized with Zn bound to it .We collected a 1.5A0
> data. Molecular Replacement didn't yield a good match for the
> protein. I want to try MAD taking advantage of the Zn atoms in
> protein. I am not sure what wavelength should i use to collect the
> diffraction data for Zn. any suggestions?
>
> Thank You
> Deepthi
>
> --
> Deepthi
>
> ......................
> Jürgen Bosch
> Johns Hopkins University
> Bloomberg School of Public Health
> Department of Biochemistry & Molecular Biology
> Johns Hopkins Malaria Research Institute
> 615 North Wolfe Street, W8708
> Baltimore, MD 21205
> Office: +1-410-614-4742
> Lab: +1-410-614-4894
> Fax: +1-410-955-2926
> http://web.mac.com/bosch_lab/
>
>
>
|