> I can't imagine the results would be very different for protein-DNA vs. protein-RNA.
The reason protein-nucleic acids is an extra category in mattprob is largely due to poorer statistics
resulting from limited sample size and hence no reliable resolution dependence can be computed.
In addition the partial specific volumes for protein (0.74 cm3/g) and nucleic acids (0.50 cm3/g) are
different, so an exact calculation needs to consider their ratio to obtain the correct psv estimate
BR
----- Original Message -----
From: "Tim Gruene" <[log in to unmask]>
To: [log in to unmask]
Sent: Monday, March 12, 2012 11:07:29 AM GMT -08:00 US/Canada Pacific
Subject: Re: [ccp4bb] Matthews coeff. from model
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Dear James,
I do not know such a tool, but you can use 140A^3/a.a. and 380A^3/base to calculate the solvent content by hand.
Regards,
Tim
On 03/12/2012 06:35 PM, james09 pruza wrote:
> Dear CCP4bbers,
>>
>> Is there any tool to calculate the Matthews coefficient from a
>> crystallographic model of RNA-protein complex?
>>
>> Thanking you.
>> James.
>>
>
- --
Dr Tim Gruene
Institut fuer anorganische Chemie
Tammannstr. 4
D-37077 Goettingen
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--
Michael C. Thompson
Graduate Student
Biochemistry & Molecular Biology Division
Department of Chemistry & Biochemistry
University of California, Los Angeles
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