Acta Cryst. (1997). D53, 734-737 [ doi:10.1107/S0907444997007233 ]
The Ultimate Wavelength for Protein Crystallography?
I. Polikarpov, A. Teplyakov and G. Oliva
http://scripts.iucr.org/cgi-bin/paper?gr0657
may give some insights.
To the OP, have you solved the structure? In some cases, seeing the packing at low resolution can give you ideas on how to change the construct to obtain higher diffracting crystals.
F
On Feb 15, 2012, at 4:21 PM, Jacob Keller wrote:
> Well, but there is more scattering with lower energy as well. The
> salient parameter should probably be scattering per damage. I remember
> reading some systematic studies a while back in which wavelength
> choice ended up being insignificant, but perhaps there is more info
> now, or perhaps I am remembering wrong?
>
> Jacob
>
> On Wed, Feb 15, 2012 at 5:14 PM, Bosch, Juergen <[log in to unmask]> wrote:
>> No impact ? Longer wavelength more absorption more damage. But between the choices given no problem.
>> Spread of spots might be better with 1.0 versus 0.9 but that depends on your cell and also how big your detector is. Given your current resolution none of the mentioned issues are deal breakers.
>>
>> Jürgen
>>
>> ......................
>> Jürgen Bosch
>> Johns Hopkins Bloomberg School of Public Health
>> Department of Biochemistry & Molecular Biology
>> Johns Hopkins Malaria Research Institute
>> 615 North Wolfe Street, W8708
>> Baltimore, MD 21205
>> Phone: +1-410-614-4742
>> Lab: +1-410-614-4894
>> Fax: +1-410-955-3655
>> http://web.mac.com/bosch_lab/
>>
>> On Feb 15, 2012, at 18:08, "Jacob Keller" <[log in to unmask]> wrote:
>>
>>> I would say the better practice would be to collect higher
>>> multiplicity/completeness, which should have a great impact on maps.
>>> Just watch out for radiation damage though. I think the wavelength
>>> will have no impact whatsoever.
>>>
>>> JPK
>>>
>>> On Wed, Feb 15, 2012 at 4:23 PM, Seungil Han <[log in to unmask]> wrote:
>>>> All,
>>>> I am curious to hear what our CCP4 community thoughts are....
>>>> I have a marginally diffracting protein crystal (3-3.5 Angstrom resolution)
>>>> and would like to squeeze in a few tenth of angstrom.
>>>> Given that I am working on crystal quality improvement, would different
>>>> wavelengths make any difference in resolution, for example 0.9 vs. 1.0
>>>> Angstrom at synchrotron?
>>>> Thanks.
>>>> Seungil
>>>>
>>>> --------------------------------------------
>>>>
>>>> Seungil Han, Ph.D.
>>>>
>>>> Pfizer Inc.
>>>>
>>>> Eastern Point Road, MS8118W-228
>>>>
>>>> Groton, CT 06340
>>>>
>>>> Tel: 860-686-1788, Fax: 860-686-2095
>>>>
>>>> Email: [log in to unmask]
>>>>
>>>>
>>>
>>>
>>>
>>> --
>>> *******************************************
>>> Jacob Pearson Keller
>>> Northwestern University
>>> Medical Scientist Training Program
>>> email: [log in to unmask]
>>> *******************************************
>
>
>
> --
> *******************************************
> Jacob Pearson Keller
> Northwestern University
> Medical Scientist Training Program
> email: [log in to unmask]
> *******************************************
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