Hi board,
Although i do not want to endorse anything personally
earlier mail to Yuris response reflects my personal reflection of what i like.
Here is a plate from Molecualr dimensions that i found
may be an alternate where you can chose the sandwich thickness
good idea but do not know what advantage it might have on
crystallization process.
link:
http://www.moleculardimensions.com//shopdisplayproducts.asp?Search=Yes
Thanks to Tony from molecular dimensions who point this out.
quote:
"We have many users of Laminex which offers a lot of flexibility
in that you can choose sandwich thickness as well as glass or plastic bases
and glass, plastic or film covers.
If you have not tested Laminex I would be pleased to send you a sample trial
pack.
Tony Savill
Molecular Dimensions Inc."
-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of Pius
Padayatti
Sent: 26 February 2012 01:08
To: [log in to unmask]
Subject: Re: [ccp4bb] Membrane Protein Crystallization Plates
Hi Yuri,
i strongly suggest these plates sold through Hampton research Paul
Marienfeld GmbH plates for your use.
here is a link , best plates in the market.
link: http://hamptonresearch.com/product_detail.aspx?cid=10&sid=182&pid=611
Plates comes with extra cover slips in addition to single glass cover for
four wells at a time whcih let you set these plates manually if you like it
that way.
Good for automations as well.
only problem: since the galss is siliconized you can not write anything on
it (may be there is some industry grade marker might work.) But there is
enough place on side to stick a printed label or bar code for
your'automation for keeping records hope this is what you wanted to know.
if you are money crunched and want to make some plates on your own. buy
regular micoscope slides, some two sided tapes (3M) very thin and a tool
that sold for cutting out small pieces of tissue samples. it looks like a
pen with sharp punch enough to make a hole in a 3M tape make a 27 hole plate
in a standard microslide.
cheers
pius
On Sat, Feb 25, 2012 at 3:35 PM, Yuri Pompeu <[log in to unmask]> wrote:
> Hello Everyone,
> I am considering the purchase of crystallization plates for membrane
proteins.
> I would love to hear what some of the community thinks or has experienced
with these.
> Particulalrly the monoolein and monoolein/cholesterol coated plates (
> I am not sure I can mention the vendor here but it "should" not matter) So
fire away. Is it worth it? Any succes stories? Bad experiences?
> I appreciate the input
> Best,
> Yuri
--
Pius S Padayatti,PhD,
Phone: 216-658-4528
--
Pius S Padayatti,PhD,
Phone: 216-658-4528
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