Actually, I would refer the ccp4-bbs to Journal of Structural Biology
175 (2011) pp216-223 for the use of fluorescence in relation to protein
crystallization.
Regards,
Bryan
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-----Original Message-----
From: CCP4 bulletin board [mailto:[log in to unmask]] On Behalf Of
Christian Roth
Sent: Tuesday, February 28, 2012 8:09 AM
To: [log in to unmask]
Subject: Re: [ccp4bb] Desalting columns
If you want test a lot of different conditions a Thermofluorecence assay
might
work for your protein and you may find a condition which stabilise your
protein. However there is no warranty that it crystallise better
afterwards.
Christian
Am Montag 27 Februar 2012 17:01:33 schrieb Sangeetha Vedula:
> Dear bb users,
>
> I am trying to crystallize a ~320 kDa protein that crashes out if
> concentrated past about 3 mg/mL.
>
> I would like to try to exchange it into various buffer-salt-additive
> combinations to see which buffer works. For a starting point, I'd like
to
> use desalting colums.
>
> Does anyone have suggestions for good buffer exchange and sample
recovery?
> I woud like to load about 250 uL onto each column.
>
> Thanks a lot!
>
> Best regards,
>
> Sangeetha.
>
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